Transgenically Encoded Protein Photoinactivation (FlAsH-FALI)
نویسندگان
چکیده
منابع مشابه
Transgenically Encoded Protein Photoinactivation (FlAsH-FALI) Acute Inactivation of Synaptotagmin I
We demonstrate a noninvasive technique for protein photoinactivation using a transgenically encoded tag. A tetracysteine motif that binds the membrane-permeable fluorescein derivative 4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein (FlAsH) was engineered into synaptotagmin I (Syt I4C). Neuronally expressed Syt I4C rescues the syt I null mutation, can be visualized after FlAsH labeling, and is no...
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ژورنال
عنوان ژورنال: Neuron
سال: 2002
ISSN: 0896-6273
DOI: 10.1016/s0896-6273(02)01068-1